Showing posts with label Transgenic Animals. Show all posts
Showing posts with label Transgenic Animals. Show all posts

Tuesday, May 21, 2013

Arachnid Alchemy - Golden Orb Spiders

This summer has certainly been a time of plenty, along with the abundant flocks of White Storks we are witnessing this year, there are also a fair number of Golden Orb Spiders. Their vast golden webs span almost every available gap between trees and shrubs. Reaching great vertical heights and stretching across distances of up to 5 m, these strong spider webs form an almost continuous network of yellow silk ready to ensnare any insect, bird or bat that is on the wing. The golden silk is thought to attract pollinators such as bees.

The big brightly coloured spider in the web is the female Nephila. She may measure 15-30 mm in length while the male is only 5 mm and weighs one thousandth of the female's weight. A web of this magnitude must require constant maintenance. The weaving of a web takes up a lot of the spider resources and orb spiders tend to eat the silk of any damaged section of web to absorb and utilize the protein. The male of this species does not produce silk but what he lacks in size and in the home maintenance department, he more than makes up for with courage and ingenuity. The female will often eat the tiny male after copulation. Where possible he presents a meal to her and while she is feeding he will copulate with his preoccupied mate. In most webs one will find the oversized female, the puny males and tiny dewdrop spiders. These small silver spiders are kleptoparasites, they patrol the web picking off the smaller prey caught therein and avoiding the owners who will eat the house guest if caught.

The webs of the Golden Orb spiders are extremely strong. The female begins a web by sending out a stand of ultra thin sticky silk into the wind. With luck this strand will stick to an upright branch. Then she will release a much thicker line allowing the wind to slide it across the first. Once the line is thick enough to carry her she will cross to the second post and continue spinning the web. The silk of the orb spiders is exceptionally elastic and can stretch up to 40% before snapping. Generally, spider silk also known as gossamer, has a tensile strength comparable to that of high-grade steel, so strong that a strand with a radius of 3 cm can apparently stop a Boeing 747. For years now research has been ongoing on how to manufacture, among other things, bullet proof vests out of spider silk. The stumbling block appears to be finding sufficient filament to weave the material. Silk is a protein, but unlike other proteins it does not decompose. We preserve other useful proteins by cooking, salting, drying etc. Spider silk has 3 ingredients that prevent its decay. Some of the proteins found in sour milk share a number of these properties. Researchers are now using a strategy called Transgenic animals to explore making synthetic spider silk. Transgenic animals are animals that have had genes from other species inserted into their genetic codes. In this case; miniature goats in New Zealand have had a silk producing gene added to their genome. Somehow the scientists anticipate producing spider silk from the goat milk of these Transgenic animals!

While all this research goes on the orb spiders have a busy time producing the golden silk and maintaining their remarkable webs. The genus name Nephila is appropriately derived from Greek, "nen" meaning "to spin" and "philos" meaning "love"- translated into "fond of spinning".

Judy Meeser lives in Greater Kruger Park and together with her husband Bruce, runs presonalised safaris in this magnificent area. They operate Mygr8 Safaris and Wildlife Encounters offering tours and safari lodges with skilled and knowledgeable guiding.

Article Source: http://EzineArticles.com/?expert=Judy_Meeser
http://EzineArticles.com/?Arachnid-Alchemy---Golden-Orb-Spiders&id=6121405

Friday, May 17, 2013

Genetically Modified Foods - Pros and Cons

When an organism's genes (its genetic materials) are changed in a laboratory, the organism is called genetically modified or transgenic. Genetically modified foods are products that contain transgenic animals or plants as ingredients. Genetically modifying food sources can make them bigger, stronger, and more nutritious. Changing genes in plants can protect them against disease. It can also help them survive when exposed to herbicides (chemicals used to kill weeds and plants) and insects. There are many uses for genetically modified foods. For example, in parts of Africa, people eat large amounts of rice. Regular rice is not very nutritious, so scientists have transgenic rice plants. These modified plants produce proteins that give their rice extra iron and vitamins. Scientists have also modified pigs to produce healthier meat and coffee plants to produce decaffeinated coffee beans, among other things.

People who support genetically altered foods argue that they can help people who live in areas with poor growing conditions. They believe these foods can help end world hunger. Those on the other side of the debate worry about the safety of genetically modified foods. They fear that mixing genes from different species could create strange new animal and plant breeds. Environmental groups worry that genetically altering foods could be dangerous to human health. They are not sure what effects genetically modified plants and animals might have on the people who eat them. Critics call these foods "Frankenfoods" because they have been pieced together using genes from different species.

One specific concern is that a gene might mistakenly be taken from a plant to which many people are allergic. For example, if a gene taken from a peanut were inserted into soybeans, it could cause the soybeans to produce peanut proteins. Those proteins could trigger a reaction in anyone who was allergic to peanuts. Another worry is that genetically modified plants might breed with the plants growing around them. Then the wild plants could pick up the traits from the modified plants. This cross-breeding could create problems such as weeds that herbicides cannot kill.

Johny Leeroy is an online publisher and webmaster. His latest site includes www.mooarcade.com, which contains a collection of over 2500 flash games. Play Online Games

Article Source: http://EzineArticles.com/?expert=Johny_Leroy
http://EzineArticles.com/?Genetically-Modified-Foods---Pros-and-Cons&id=4480176

Thursday, May 16, 2013

Pharm Goats and Malaria

The people at A&M College of Veterinary Medicine and Texas Agrilife Research have a dream. They envision people in impoverished countries going out to milk their goat and from the milk get a vaccine for malaria and possibly other diseases as well, or perhaps obtaining important drugs. It is a good idea because goats are hardy animals that can thrive in impoverished areas. Their milk provides good nutrition, and now perhaps can provide much more. This is the latest advance in transgenic research.

What is Pharming?

The term pharming was adopted to mean the production of human drugs in farm animals. This is not new research. On the contrary, research on transgenic animals began in the 1980s. The first product was Humulin, which is human insulin produced by bacteria and it was produced in 1982. There are several human gene products that have been studied, including Factor IX, insulin, growth hormone, a-1-antitrypsin, antithrombin III and tissue plasminogen activator. The last two were studied in the goat.

How does it work?

The goat's genes relating to milk production are manipulated so that they produce the malaria antigen, through the creation of what is called a transgenic goat.

A simplified explanation is that through genetic manipulation, the DNA is cut removing the sequence that is of interest and then this sequence is joined with a vector, in order to transfer it to another organism. There are actually several techniques that are employed to accomplish the genetic transfer. One older method uses DNA microinjection of fertilized eggs, while a newer method uses whole nuclear transfer.

The Controversy

This subject is a hotbed of controversy. There are people on both sides of the issue and both sides are passionate about their views. The concern that people feel over genetic manipulation and the dangers that it poses, has even been translated onto the silver screen in movies like Resident Evil series and Gattaca, among many others.

There are many who are against transgenic work in both animals and crops. People worry that we are going too far and we do not understand the consequences of genetic manipulation. Also, that we do not and cannot fully understand what will be the result of this manipulation.

Many people are against genetic manipulation of crops and animals, thus the Organic movement was born. People are willing to pay extra to buy vegetables and animals that are free of drugs and genetic manipulation.

On the other hand, this and similar research can help millions in impoverished areas that suffer from disease, such as malaria and cannot afford vaccinations. This extends to other drugs that can be produced in animals, including goats. In the future, whether entire human organs can successfully be grown in animals is yet to be proven. How many people could be saved?

Genetic manipulation of crops has led to better and higher production rates, because these crops have been genetically modified to have better resistance to pests and diseases. Higher yields translate into more food available for people who are starving.

http://www.goatlapshop.com Web site dedicated to goats and their welfare.

Felicity McCullough latest publications:

Goat Knowledge Series are: -

How To Keep Goats Healthy; Golden Guernsey Goats; A Simple Guide To The Goat's Digestive System; Managing Goat Nutrition What You Need To Know A Simple Guide; Success Guide For Raising Healthy Goats

Other articles in the series:

The Fun of Goats; Schmallenberg Virus Outbreak: Focus On Goats And The UK

Article Source: http://EzineArticles.com/?expert=Felicity_McCullough
http://EzineArticles.com/?Pharm-Goats-and-Malaria&id=6953421

Wednesday, May 15, 2013

Insulin Production From the Milk of Argentine Cow Clones

1. INTRODUCTION:

1.1 Insulin

Insulin is a peptide hormone made of 51 amino acids composing two chains A and B. The A chain has 21 amino acids and the B chain has 30 amino acids. The two chains are linked by intra and inter disulphide linkages. The release of this hormone is mainly initiated by the blood glucose levels.

1.2 Diabetes Mellitus

The inability of the 2-cells of Langerhans to secrete adequate or inability to secrete insulin following the glucose load is said to be Diabetes mellitus. The complications of diabetes are Cataract formation, Acute to Chronic renal failure, Cardiac problems, unhealed wounds, Mycoses etc.

Due to the above said complications, it has impacts on the living standards of people. The worldwide diabetic population is about 200 million. The WHO data reveal that this will become doubled by 2025 ( based on 2002 data ). This implies the importance of this hormone.

1.3 History of Insulin

Baunting and Best developed the use of insulin therapy in 1921. Insulin was the first protein to be sequenced by Frederick Sanger in 1950s. For about 60 years diabetics were dependent on natural sources of insulin with attendant problems of supply and quality. In the late 1970s and early 1980s recombinant DNA technology enabled scientists to synthesis insulin in bacteria.

The best natural source of insulin is human insulin which can be isolated in crystalline form from the cadaver of human. It costs approximately about 5000$ per vial, which is practically impossible. As diabetes affects irrational of sex, race, economic status which led scientists to think of alternate techniques that will bring down the production cost. Using plasmid vectors, scientists produce insulin from E.coli by rDNA technology. It has its own advantages and disadvantages. It has low generation time but the chances for contamination are high.

2. TRANSGENIC ANIMALS

Generation of transgenic animals is complex in terms of both technical difficulty and ethical problems.

2.1 Utilisation of Transgenic Animals to Produce Proteins

The use of transgenic animals to produce the proteins of human interest was already in practice. One of such example is production of tissue Plasminogen Activator (tPA) in the milk of goats. Here the mammary control DNA and coding DNA for tissue plasminogen activator are utilized to produce rDNA. The hybrid gene is inoculated in to fertilized egg ( isolated from a goat ) by microinjection. The microinjected fertilized egg is transferred to a foster mother. Then the hybrid gene carriers were mate to produce the transgenic female homozygous for the transgene. This transgenic technology enables goats to secrete tPA in milk. A similar technique with little modifications is used to make cows secrete insulin in milk. 2.2 Procedure

Here the animal selected is Jersey heifer which is known for its abundant milk production. The mammary control DNA of Jersey heifer fetus is isolated. In animals and Plants, the DNA to Protein ratio is less. Hence the nuclei isolated first. This increases the ratio of DNA to Protein and avoids contamination of chromosomal DNA by DNA from cytoplasmic organelles. The nuclei opened , the RNA and Protein are enzymatically digested, then the DNA is precipitated.

o The coding DNA for human insulin is isolated in the same manner.

o It is then treated with type II Restriction Endonucleases to cut at specific sites.

o The required DNA sequences are joined together using DNA ligase enzymes.

o The hybrid gene is introduced in to the cell by microinjection. Once the gene enters the cell should enter the nucleus.

o The Jersey heifer's eggs are taken and the nucleus is removed using a micropipette.

o The genetically modified nuclei are fused with enucleated eggs using cloning techniques.

o The electrical stimulus cause cell dividing and an early embryo is developed.

o The embryo cells are separated and are implanted in surrogate mother cows.

o It gives rise to 4 genetically modified calves in 385 ± 5 days.

o These calves will reach maturity in 18 - 24 months at which they are capable of producing milk.

o Once they start producing milk, the insulin can be obtained by purification and refining of milk using protein purification techniques like HPLC.

- Scientists isolated the specific cell types from Jersey heifer's fetus from a slaughter house

- The rDNA is introduced in to the cell which reaches the nucleus

- The genetically modified nuclei is fused with enucleated cattle eggs using cloning techniques

- The electrical impulse starts cell division

- The cells are individualized and can be implanted into 4 surrogate mother cows

- The mother cow will give birth to genetically modified calves in 385 ± 5 days

- The genetically modified calves will reach adulthood in 18 - 24 months

- Once they start milk production, the insulin can be obtained by purification and refining of milk.

3. Conclusion

o This technique will definitely can reduce the production cost by atleast 30%.

o The complications can be overcome by further working with this.

o This will definitely cause a revolution in the utilization of transgenic animals for protein production if the usual difficulties are solved.

4. BIBLIOGRAPHY

Google search

BIOTECHNOLOGY Mohan. P. Arora ( 2004) Himalaya Publishing House

GENETIC ENGINEERING Desmond S. T. Nicholl, Paisley ( 2002 ) Cambridge University Press

MOLECULAR BIOTECHNOLOGY- Principles and Applications of Recombinant DNA Bernard R. Glick and Jack J. Pasternak ( 2002 )

Article Source: http://EzineArticles.com/?expert=Abdul_Rasheed_S
http://EzineArticles.com/?Insulin-Production-From-the-Milk-of-Argentine-Cow-Clones&id=1925683

Friday, April 12, 2013